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神経向性マウス肝炎ウイルスに感染したマウスの脊髄にマウス神経幹細胞の外科的移植
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Surgical Transplantation of Mouse Neural Stem Cells into the Spinal Cords of Mice Infected with Neurotropic Mouse Hepatitis Virus
Please note that all translations are automatically generated.
Click here for the English version.
神経向性マウス肝炎ウイルスに感染したマウスの脊髄にマウス神経幹細胞の外科的移植
DOI:
10.3791/2834-v
•
08:33 min
•
July 10, 2011
•
Kevin S. Carbajal
2
,
Jason G. Weinger
2
,
Lucia M. Whitman
2
,
Chris S. Schaumburg
2
,
Thomas E. Lane
2,3
1
Department of Molecular Biology and Biochemistry
,
University of California, Irvine
,
2
Sue and Bill Gross Stem Cell Center
,
University of California, Irvine
,
3
Institute for Immunology
,
University of California, Irvine
Chapters
00:05
Title
01:25
Preparation for Surgery and Cells for Transplant
02:54
Surgery and Transplantation
04:45
Injection of Cells
07:00
Sutures and Post-op Care
07:53
Successful Spinal Cord Transplantation
08:07
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
確立された脱髄とマウスの脊髄にマウス神経幹細胞(NSC)の移植が詳述されます。 NSCの準備、胸椎9(T9)の椎弓切除、そしてNSCの移植は、マウスの前と術後ケアと一緒に概説されています。
Tags
Surgical Transplantation
Mouse Neural Stem Cells
Spinal Cords
Mice Infected
Neurotropic Mouse Hepatitis Virus
JHM Strain
Demyelinating Disease
Multiple Sclerosis
Transplantation Of NSCs
Remyelination
Clinical Outcome
Cell Replacement Therapies
Chronic Neurologic Diseases
In Vivo Models
Engrafted Cells
Adapted Method
I) Preparation Of NSCs
Ii) Pre-operative Care Of Mice
Iii) Exposure Of The Spinal Cord Via Laminectomy
Iv) Stereotactic Injection Of NSCs
Iv) Post-operative Care
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