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无试剂检测核酸,蛋白质和小分子的电化学DNA生物传感器的制备
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal
Bioengineering
Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules
Please note that all translations are automatically generated.
Click here for the English version.
无试剂检测核酸,蛋白质和小分子的电化学DNA生物传感器的制备
DOI:
10.3791/2922-v
•
13:15 min
•
June 01, 2011
•
Aaron A. Rowe
,
Ryan J. White
,
Andrew J. Bonham
,
Kevin W. Plaxco
1
Department of Chemistry and Biochemistry
,
University Of California Santa Barbara
,
2
Department of Chemistry and Biochemistry, Program in BioMolecular Science and Engineering
,
University Of California Santa Barbara
Chapters
00:05
Title
01:28
Preparation of Chemically Modified Probe DNA
02:48
Sensor Preparation
05:30
Sensor Testing, DNA Detection
07:57
Sensor Testing, Antibody Detection
09:47
Sensor Testing, Small Molecule Detection
11:24
Representative Results obtained using Electrochemical-DNA Biosensors
12:27
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
无试剂,“E - DNA”的传感器,电化学传感器表现良好,即使在血液和其他复杂的矩阵直接挑战时,已适应了广泛的核酸,蛋白质和小分子分析物的检测。在这里,我们介绍了这种传感器的制造和使用的一般程序。
Tags
Electrochemical-DNA Biosensors
Reagentless Detection
Nucleic Acids
Proteins
Small Molecules
Bedside Tests
Rapid Detection
Quantitative Detection
Clinical Samples
Environmental Samples
E-DNA Biosensors
Target DNA Sequence Detection
Polymerase Chain Reaction Mixture
HIV-specific Antibody Detection
Drug Cocaine Detection
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