JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Immunology and Infection
/
体外
广角T细胞在小鼠淋巴结切片和共聚焦显微镜成像
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Ex vivo
Imaging of T Cells in Murine Lymph Node Slices with Widefield and Confocal Microscopes
Please note that all translations are automatically generated.
Click here for the English version.
体外
广角T细胞在小鼠淋巴结切片和共聚焦显微镜成像
DOI:
10.3791/3054-v
•
09:45 min
•
July 15, 2011
•
Hélène Salmon
2
,
Ana Rivas-Caicedo
2
,
François Asperti-Boursin
2
,
Camille Lebugle
2
,
Pierre Bourdoncle
2
,
Emmanuel Donnadieu
2
1
Institut Cochin
,
Université Paris Descartes, CNRS (UMR 8104)
,
2
Inserm
,
U1016, Paris, France
Chapters
00:05
Title
01:18
Preparing Slices from Lymph Nodes
03:58
Isolation and Labeling of T cells from Mouse Lymph Nodes
05:08
Plating Labeled T cells onto Lymph Node Slices
05:45
Preparation of Microscope and Lymph Node Slices for Imaging
06:27
Imaging T cells with a Widefield Inverted Microscope
07:24
Imaging T cells with a Confocal Upright Microscope
07:53
Representative Images and Movies of T cell Accumulation, Recruitment, and Motility within Lymph Node Slices
09:17
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
这个协议描述了一种方法引入到淋巴结切片的荧光图像的T细胞。该技术允许传统的宽视场荧光或共聚焦显微镜的实时分析,T细胞迁移。
Tags
Ex Vivo Imaging
T Cells
Lymph Node Slices
Widefield Microscope
Confocal Microscope
Migration Of T Cells
Chemokines
Two-photon Microscopy
Quantitative Information
Interactions With Other Cells
Slice Assay
Fluorescently Labeled T Cells
Acutely Prepared Lymph Node Slice
Real-time Imaging
Natural Environment
Mechanisms Underlying T Cell Migration
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
T细胞受体在小鼠T细胞的逆转录病毒转导
前
肿瘤反应性T细胞
的体外
扩增Bryostatin 1/Ionomycin手段和共同的γ链细胞因子配方
腹股沟淋巴结的活体显微镜
小鼠浅表淋巴结手术
小鼠腘窝淋巴结的活体成像
淋巴结脂肪垫对嵌合体的淋巴结基质细胞起源研究的一代
评估的HIV-1感染的CD4的先天传感<sup> +</sup>性T细胞浆细胞样树突细胞使用<em>体外</em>共培养体系。
鼠淋巴结基质细胞的分离
一个<em>体外</em>急性脑片的少突胶质细胞定向T细胞攻击模式
诱导小鼠肠道炎症由效应CD4的过继转移<sup> +</sup> CD45RB<sup>高</sup> T细胞免疫缺陷小鼠
Read Article