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Journal
/
Immunology and Infection
/
広視野と共焦点顕微鏡によるマウスリンパ節スライスにおけるT細胞の
ex vivoでの
イメージング
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Ex vivo
Imaging of T Cells in Murine Lymph Node Slices with Widefield and Confocal Microscopes
Please note that all translations are automatically generated.
Click here for the English version.
広視野と共焦点顕微鏡によるマウスリンパ節スライスにおけるT細胞の
ex vivoでの
イメージング
DOI:
10.3791/3054-v
•
09:45 min
•
July 15, 2011
•
Hélène Salmon
2
,
Ana Rivas-Caicedo
2
,
François Asperti-Boursin
2
,
Camille Lebugle
2
,
Pierre Bourdoncle
2
,
Emmanuel Donnadieu
2
1
Institut Cochin
,
Université Paris Descartes, CNRS (UMR 8104)
,
2
Inserm
,
U1016, Paris, France
Chapters
00:05
Title
01:18
Preparing Slices from Lymph Nodes
03:58
Isolation and Labeling of T cells from Mouse Lymph Nodes
05:08
Plating Labeled T cells onto Lymph Node Slices
05:45
Preparation of Microscope and Lymph Node Slices for Imaging
06:27
Imaging T cells with a Widefield Inverted Microscope
07:24
Imaging T cells with a Confocal Upright Microscope
07:53
Representative Images and Movies of T cell Accumulation, Recruitment, and Motility within Lymph Node Slices
09:17
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
このプロトコルは、リンパ節のスライスに導入された画像蛍光T細胞への方法を説明します。技術は、従来の広視野蛍光または共焦点顕微鏡でのT細胞遊走のリアルタイム分析を可能にします。
Tags
Ex Vivo Imaging
T Cells
Lymph Node Slices
Widefield Microscope
Confocal Microscope
Migration Of T Cells
Chemokines
Two-photon Microscopy
Quantitative Information
Interactions With Other Cells
Slice Assay
Fluorescently Labeled T Cells
Acutely Prepared Lymph Node Slice
Real-time Imaging
Natural Environment
Mechanisms Underlying T Cell Migration
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