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基因组编辑与的CompoZr自锌指核酸酶(ZFNs)
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Genome Editing with CompoZr Custom Zinc Finger Nucleases (ZFNs)
Please note that all translations are automatically generated.
Click here for the English version.
基因组编辑与的CompoZr自锌指核酸酶(ZFNs)
DOI:
10.3791/3304-v
•
09:11 min
•
June 14, 2012
•
Keith Hansen
,
Matthew J. Coussens
,
Jack Sago
,
Shilpi Subramanian
,
Monika Gjoka
,
Dave Briner
1
Emerging Technologies
,
Sigma Life Science
Chapters
00:05
Title
01:36
Initiating the ZFN Design Process
03:23
Custom ZFN Production and Validation
04:16
Delivery of Validated ZFNs by Nucleofection
06:25
Cel-1 Assay
07:09
Clonal Isolation and Characterization
07:46
Estimated ZFN Activity from the Cel-1 Assay
08:27
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
CompoZr自锌指核酸酶(ZFN)服务可在任何生物体或细胞系在由用户定义的任何轨迹的精确的基因组编辑。本文描述的CompoZr定制ZFN服务的设计,制造,验证和实施的过程。
Tags
Genome Editing
CompoZr Custom Zinc Finger Nucleases
Gene Function
Genetic Basis Of Disease
Traditional Gene Editing Methods
Chemical-based Mutagenesis
Random Integration Of DNA Sequences
Synthetic Sequences
Aberrant Culture Conditions
Transient ZFN Expression
Precise Gene Editing
Highly Efficient Manner
Zinc Finger Nucleases
Double-stranded DNA (dsDNA)
CompoZr ZFN Unit
DNA-cleavage Domains
Double-strand Break (DSB)
Non-homologous End-joining (NHEJ) DNA Repair Pathway
Small Insertions And Deletions (indels)
Frameshift
Functional Knockout
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