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Silencing the Spark: CRISPR/Cas9 Genome Editing in Weakly Electric Fish
Silencing the Spark: CRISPR/Cas9 Genome Editing in Weakly Electric Fish
JoVE Journal
Biology
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JoVE Journal Biology
Silencing the Spark: CRISPR/Cas9 Genome Editing in Weakly Electric Fish

Silencing the Spark: CRISPR/Cas9 Genome Editing in Weakly Electric Fish

9,916 Views

08:00 min

October 27, 2019

DOI:

08:00 min
October 27, 2019

9877 Views
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Here, a protocol is presented to produce and rear CRISPR/Cas9 genome knockout electric fish. Outlined in detail are the required molecular biology, breeding, and husbandry requirements for both a gymnotiform and a mormyrid, and injection techniques to produce Cas9-induced indel F0 larvae.

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