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Silencing the Spark
 

Silencing the Spark: CRISPR/Cas9 Genome Editing in Weakly Electric Fish

Article DOI: 10.3791/60253-v
October 27th, 2019

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Summary October 27th, 2019

Here, a protocol is presented to produce and rear CRISPR/Cas9 genome knockout electric fish. Outlined in detail are the required molecular biology, breeding, and husbandry requirements for both a gymnotiform and a mormyrid, and injection techniques to produce Cas9-induced indel F0 larvae.

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