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Silencing the Spark
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Silencing the Spark: CRISPR/Cas9 Genome Editing in Weakly Electric Fish

Article DOI: 10.3791/60253-v • 08:00 min • October 27th, 2019
October 27th, 2019 •
1, 2, 2, 3, 1
1Department of Integrative Biology, Michigan State University, 2Faculty of Life Sciences, Unit of Biology and Ecology of Fishes, Humboldt University, 3Department of Biology, Cape Breton University

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Summary
Automatic Translation

Here, a protocol is presented to produce and rear CRISPR/Cas9 genome knockout electric fish. Outlined in detail are the required molecular biology, breeding, and husbandry requirements for both a gymnotiform and a mormyrid, and injection techniques to produce Cas9-induced indel F0 larvae.

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CRISPR/Cas9 Genome Editing Weakly Electric Fish Comparative Context Specific Genes Genetic Variance Phenotypic Variation Mutant Embryos Convergently Evolved Species Transcriptomic Resources Genomic Resources SgRNAs Microinjections 3D Visualization 2D Plane Zebrafish Colony General Microinjection Techniques Savvas Constantinou Jared Thompson Breeding Conditions Swollen Gonads Swollen Bellies Deep-bodied Appearance Anesthesia Administration Spawning Agent
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