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多色揭示神经回路地形

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Revealing Neural Circuit Topography in Multi-Color

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多色揭示神经回路地形

DOI:

•

09:11 min

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November 14, 2011

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Stacey L. Reeber, Samrawit A. Gebre, Nika Filatova, Roy V. Sillitoe

¹Dominick P. Purpura Department of Neuroscience, Albert Einstein College of Medicine,Yeshiva University

Chapters

00:05Title
01:38Preparing the Animal for Sterile Surgery
02:53Delivering Tracers in vivo Using Sterile Surgery
05:31Neural Circuit Topography of the Cerebellum
08:31Conclusion

Summary

Automatic Translation

我们提供了一个实用指南提供示踪剂<em>在体内</em>，并使用作为一个模型系统的脊髓小脑通路证明成功在小鼠神经元电路分析的基本步骤。我们详细描述了我们全方位的跟踪协议，它利用麦胚凝集素（WGA）的共轭Alexa的荧光团。

Tags

Neural Circuit Topography Multi-color Neural Circuits Functional Topographic Maps Visualizing Circuit Architecture Labeling Topographic Projections Cerebellum Spinocerebellar Mossy Fibers Wheat Germ Agglutinin (WGA)Alexa 555 Alexa 488 Tracing Neural Projections Retrograde Transport Anterograde Transport Tracers Preparation Spinocerebellar Tracing Surgery Three-dimensional Imaging Functional Maps Organization Connectivity

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