A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 01:00Dissection of the Cerebellum
- 02:05Processing the Tissue for Wholemount Staining
- 06:10Imaging the Stained Tissue
- 06:49Results: Zebrin II and CART Immunohistochemistry in Wholemount Cerebella
- 08:55Conclusion
Neural circuits are topographically organized into functional compartments with specific molecular profiles. Here, we provide the practical and technical steps for revealing global brain topography using a versatile wholemount immunohistochemical staining approach. We demonstrate the utility of the method using the well-understood cytoarchitecture and circuitry of cerebellum.
Tags
Wholemount ImmunohistochemistryBrain TopographyCerebellumCellular ArchitectureGene Expression DomainsProtein Expression DomainsMolecular CompartmentalizationAfferent FibersPatterning DefectsMouse CerebellumWholemount Staining ApproachProtein Expression PatternsZebrinII/aldolaseC ExpressionThree-dimensional ConformationAfferent ProjectionsComparative StudiesMolecular TopographyRat Cerebellum
