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JoVE Journal
Neuroscience

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Wholemount Immunohistochemistry for Revealing Complex Brain Topography
 

Wholemount Immunohistochemistry for Revealing Complex Brain Topography

Article DOI: 10.3791/4042
April 5th, 2012

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Summary April 5th, 2012

Neural circuits are topographically organized into functional compartments with specific molecular profiles. Here, we provide the practical and technical steps for revealing global brain topography using a versatile wholemount immunohistochemical staining approach. We demonstrate the utility of the method using the well-understood cytoarchitecture and circuitry of cerebellum.

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