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Chapters
- 00:05Title
- 01:38Preparing the Animal for Sterile Surgery
- 02:53Delivering Tracers in vivo Using Sterile Surgery
- 05:31Neural Circuit Topography of the Cerebellum
- 08:31Conclusion
Vi tilbyder en praktisk guide til at levere sporstoffer<em> In vivo</em> Og bruge spinocerebellar sti som en model for at demonstrere væsentlige skridt for en vellykket neurale kredsløb analyse hos mus. Vi beskriver i detaljer vores alsidige tracing-protokol, der udnytter hvedekim agglutinin (WGA) konjugeret til Alexa fluorophores.
Tags
Neural Circuit TopographyMulti-color Neural CircuitsFunctional Topographic MapsVisualizing Circuit ArchitectureLabeling Topographic ProjectionsCerebellumSpinocerebellar Mossy FibersWheat Germ Agglutinin (WGA)Alexa 555Alexa 488Tracing Neural ProjectionsRetrograde TransportAnterograde TransportTracers PreparationSpinocerebellar Tracing SurgeryThree-dimensional ImagingFunctional Maps OrganizationConnectivity
