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New Tools to Expand Regulatory T Cells from HIV-1-infected Individuals
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Immunology and Infection
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JoVE Journal Immunology and Infection
New Tools to Expand Regulatory T Cells from HIV-1-infected Individuals

New Tools to Expand Regulatory T Cells from HIV-1-infected Individuals

DOI:
10.3791/50244-v

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09:27 min

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May 30, 2013

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, ,
1Ragon Institute of MGH, MIT, and Harvard, 2Division of Infectious Diseases,Massachusetts General Hospital

Chapters

  • 00:05Title
  • 01:44Regulatory T cell isolation from HIV-1 Positive Blood
  • 03:40Cell Culture
  • 04:27Phenotyping
  • 05:14Suppression Assay
  • 07:18Results: Representative Treg Cells Expanded from HIV-1-infected Individuals
  • 08:33Conclusion

Summary

Automatic Translation

Automatic Translation

CD4+ Regulatory T cells are potent immune-modulators and serve important functions in immune homeostasis. The paucity of these cells in peripheral blood makes functional studies challenging, specifically in the context of HIV-1-infection. We here describe a method to isolate and expand functional CD4+ Tregs from peripheral blood from HIV-1-infected individuals.

Tags

Keywords: HIV-1Regulatory T CellsTregsCD4+ T CellsImmune ModulationImmunopathogenesisExpansionIn VitroFlow CytometryFOXP3CTLA4HELIOSSuppression

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