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在体外对HIV - 1核心脱壳
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
In vitro Uncoating of HIV-1 Cores
DOI:

10:49 min

November 08, 2011

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Chapters

  • 00:05Title
  • 01:14Production of HIV-1 Particles
  • 03:04Concentration of HIV-1 Virions by Ultracentrifugation
  • 04:30Sucrose Gradient Centrifugation to Isolate Cores
  • 06:36Kinetic Assay of HIV-1 Uncoating
  • 10:21Conclusion

Summary

Automatic Translation

脱壳是在HIV - 1生命周期的早期阶段的重要一步,并定义为衣壳外壳的拆卸和释放病毒核蛋白复合物(vRNP)。在这里,我们展示了隔离HIV - 1病毒颗粒的完整的核心和量化其脱壳技术<em>在体外</em>。

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