Journal
/
Immunology and Infection
/
在体外对HIV - 1核心脱壳
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
In vitro Uncoating of HIV-1 Cores
Please note that all translations are automatically generated. Click here for the English version.

在体外对HIV - 1核心脱壳

DOI:
10.3791/3384-v

•

10:49 min

•

November 08, 2011

•

,
1Department of Pathology, Microbiology and Immunology,Vanderbilt University School of Medicine

Chapters

  • 00:05Title
  • 01:14Production of HIV-1 Particles
  • 03:04Concentration of HIV-1 Virions by Ultracentrifugation
  • 04:30Sucrose Gradient Centrifugation to Isolate Cores
  • 06:36Kinetic Assay of HIV-1 Uncoating
  • 10:21Conclusion

Summary

Automatic Translation

Automatic Translation

脱壳是在HIV - 1生命周期的早期阶段的重要一步，并定义为衣壳外壳的拆卸和释放病毒核蛋白复合物（vRNP）。在这里，我们展示了隔离HIV - 1病毒颗粒的完整的核心和量化其脱壳技术<em>在体外</em>。

Tags

In Vitro UncoatingHIV-1 CoresRetrovirusesCapsidLipid EnvelopeLentivirusesCA ProteinHexagon LatticePentamersCone StructureViral Ribonucleoprotein ComplexFusionTarget Cell MembraneCytoplasmDisassemblySoluble CAUncoating ProcessIntracellular LocationTimingAmino-acid SubstitutionsStability Of The CapsidHIV-1 InfectionAssaysQuantitative MethodIsolation Of CoresSedimentationConcentrated VirionsDetergent LayerSucrose Gradient

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

V3的HIV - 1取向的基因型推理使用人口为基础的测序

艾滋病毒的成像1信封引起的病毒学突触信号合成脂双层

一个<em>在体外</em>共感染模型研究<em>恶性疟原虫</em人原发性单核细胞源性的免疫细胞-HIV-1相互作用

新工具展开调节性T细胞的HIV-1感染的个体

限制性内切酶克隆方法，以评估<em>在体外</em> HIV-1 C亚型的Gag-MJ4嵌合病毒的复制能力

HIV-1的三聚体包膜糖蛋白的构象评估使用基于细胞的ELISA检测

从HIV-1阳性者的血浆分离外来体的

HIV-1 Gag的可视化绑定到巨人的单片囊泡（GUV）膜

RNA的疗效和毒性的评价基因或药物治疗靶向HIV-1的生产使用

吞噬体偏移和速度与HIV-1感染的活小学的人巨噬细胞测量

Read Article