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Chapters
- 00:05Title
- 02:50Enzymatic Isolation of Tomato Cuticles
- 03:44Exhaustive Dewaxing by Soxhlet Extraction
- 05:12Molecular Characterization of Tomato Fruit Cutin by CPMAS ssNMR
- 07:17Results: CPMAS ssNMR Analysis of Isolated Cutin Protective Polymer
- 08:11Selective Isolation of Epicuticular and Intracuticular Waxes
- 09:32Probing the Tomato Cuticle Surface with Atomic Force Microscopy (AFM)
- 14:02Results: CPMAS ssNMR and AFM Analysis of Selectively Dewaxed Tomato Fruit Cuticles
- 14:43Conclusion
Aerial plant organs are protected by the cuticle, a supramolecular biopolyester-wax assembly. We present protocols to monitor selective removal of epi- and intracuticular waxes from tomato fruit cuticles on molecular and micro scales by solid-state NMR and atomic force microscopy, respectively, and to assess the cross-linking capacity of engineered cuticular biopolyesters.
Tags
IsolationBiophysical StudyFruit CuticlesHydrophobic Protective LayerTerrestrial PlantsDefensive BarrierBiotic StressAbiotic StressWater FlowExternal EnvironmentCutinLong-chain Fatty AcidsWaxesStructural FrameworkCuticular LayerEpicuticular LayerIntracuticular WaxesComprehensive ProtocolCommercial Tomato Fruit CuticlesSequential Wax RemovalSolid-state Cross-polarization Magic-angle-spinning (CPMAS) 13C NMR SpectroscopyAtomic Force Microscopy (AFM)Molecular-level Structural ProfilesBulk MaterialsMicroscale TopographyRoughnessCuticular SurfacesCross-linking Capabilities
