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Journal
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Biology
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大鼠门户成纤维细胞的分离<em>原位</em>肝脏灌注
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Isolation of Rat Portal Fibroblasts by
In situ
Liver Perfusion
Please note that all translations are automatically generated.
Click here for the English version.
大鼠门户成纤维细胞的分离<em>原位</em>肝脏灌注
DOI:
10.3791/3669-v
•
07:39 min
•
June 29, 2012
•
Jessica W. Wen
,
Abby L. Olsen
,
Maryna Perepelyuk
,
Rebecca G. Wells
1
Division of Gastroenterology, Hepatology & Nutrition, Department of Pediatrics
,
The Children’s Hospital of Philadelphia
,
2
Department of Medicine
,
University of Pennsylvania
Chapters
00:05
Title
01:20
Perfusion of the Liver
03:31
Preparation of the Biliary Tree and Isolation of the Portal Fibroblast Fraction
06:42
Results: Representative Primary Portal Fibroblasts Isolated from Adult Rat Liver
07:21
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
一个从大鼠肝门成纤维细胞的分离技术。肝脏被灌注和消化<em>原位</em>胶原酶,其次是<em>体外</em>消化肝细胞浆和大小选择。这种方法提供了一个门户网站的成纤维细胞的纯人口,而不需要通过文化。
Tags
Isolation
Rat Portal Fibroblasts
In Situ Liver Perfusion
Liver Fibrosis
Extracellular Matrix
Activated Myofibroblasts
Hepatic Stellate Cells
Bone Marrow Derived Fibroblasts
Biliary Fibrosis
Biliary Epithelial Injury
Scarring
In Vitro Study
Cell Population
Isolation Technique
Outgrowth Technique
Liver Perfusion
Enzymatic Digestion
Size Selection
Primary Cells
Article
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