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Chapters
- 00:05Title
- 00:55Heart Langendorff Perfusion and Digestion
- 03:43Cardiomyocyte Isolation
- 05:08Endothelial Cell and Fibroblast Isolation
- 07:45Results: Representative Cardiac Cell Analyses
- 08:25Conclusion
Several protocols have been developed and described for the isolation of different cardiac cell types from a rat heart. Here, an optimized protocol is described that allows the isolation of high-quality major cardiac cell types (cardiomyocytes, endothelial cells, and fibroblasts) from a single preparation, reducing the experimental costs.
