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微型计算机断层扫描成像制备小鼠冠状动脉血管逆行灌注和灌装

JoVE Journal
Medicine

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JoVE Journal Medicine

Retrograde Perfusion and Filling of Mouse Coronary Vasculature as Preparation for Micro Computed Tomography Imaging

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微型计算机断层扫描成像制备小鼠冠状动脉血管逆行灌注和灌装

DOI:

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10:16 min

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February 10, 2012

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Jill J. Weyers, Dara D. Carlson, Charles E. Murry², Stephen M. Schwartz, William M. Mahoney, Jr.

¹Department of Pathology, Center for Cardiovascular Biology, and Institute for Stem Cell and Regenerative Medicine,University of Washington, ²Departments of Bioengineering and Medicine/Cardiology,University of Washington

Chapters

00:05Title
01:27Equipment Preparation
02:18Exposing the Heart and Cannulating the Aorta
04:19Perfusion and Microfil Injection
08:16Representative Microfilled Hearts
09:45Conclusion

Summary

Automatic Translation

冠状动脉血管的可视化来推进我们的心血管疾病的认识是至关重要的。在这里，我们描述了在微型计算机断层扫描成像（μCT）准备，与不透X线的硅橡胶（Microfil）灌注小鼠冠状动脉血管的方法。

Tags

Retrograde Perfusion Filling Mouse Coronary Vasculature Micro Computed Tomography Imaging Visualization Imaging Techniques Vascular Network Resolution Vascular Casting Techniques Micro-Computed Tomography (µCT)Radiopaque Compound Accurate Representation Filling Technique In Vivo Cannulation Retrograde Perfusion Langendorff Perfusion System

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