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这些模型在体外间皮间隙检测卵巢癌转移的早期步骤

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Medicine

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JoVE Journal Medicine

In vitro Mesothelial Clearance Assay that Models the Early Steps of Ovarian Cancer Metastasis

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这些模型在体外间皮间隙检测卵巢癌转移的早期步骤

DOI:

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08:54 min

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February 17, 2012

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Rachel A. Davidowitz, Marcin P. Iwanicki, Joan S. Brugge

¹Department of Cell Biology,Harvard Medical School

Chapters

00:05Title
01:22Ovarian Cancer Cell Spheroid Formation
03:28Mesothelial Cell Monolayer Formation
06:15Results: Analysis of Mesothelial Clearance by Ovarian Cancer Cell Spheroids
08:32Conclusion

Summary

Automatic Translation

这里描述的皮间隙检测，利用荧光标记的细胞和延时视频显微镜，可视化和定量测量卵巢癌多细胞球体和间皮细胞单层的相互作用。此法模拟卵巢癌转移的早期步骤。

Tags

In Vitro Mesothelial Clearance Assay Ovarian Cancer Metastasis Secondary Metastases Peritoneal Cavity Multicellular Clusters Spheroids Mesothelial Cells Peritoneal Tumor Masses Extracellular Matrix Primary Ovarian Cancer Cells Primary Peritoneal Mesothelial Cells

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