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JoVE Journal Bioengineering
Visualisering af Cortex Organisation og Dynamics i mikroorganismer, ved hjælp af total indre refleksion Fluorescens Mikroskopi
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Visualisering af Cortex Organisation og Dynamics i mikroorganismer, ved hjælp af total indre refleksion Fluorescens Mikroskopi

Article DOI: 10.3791/3982-v • 14:15 min • May 1st, 2012
May 1st, 2012 •
1, 1, 1,2, 1
1AG Cellular Dynamics and Cell Patterning, Max Planck Institute of Biochemistry, 2Helmholtz Zentrum München

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Total indre refleksion Fluorescens (TIRF) mikroskopi er en kraftfuld fremgangsmåde til at observere strukturer tæt på celleoverfladen ved høj kontrast og tidsmæssig opløsning. Vi viser, hvordan TIRF kan anvendes til at studere protein dynamik ved cortex af cellevæg-lukkede bakterielle og fungale celler.

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Bioengineering TIRF billeddannelse gær bakterier mikroskopi celle cortex
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