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绿色怪物承载多基因缺失的酵母菌株的产生过程
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JoVE Journal Biology
The Green Monster Process for the Generation of Yeast Strains Carrying Multiple Gene Deletions
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绿色怪物承载多基因缺失的酵母菌株的产生过程

DOI:
10.3791/4072-v

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13:06 min

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December 15, 2012

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, , , , ,
1Department of Synthetic Biology and Bioenergy,J. Craig Venter Institute, 2Department of Microbial and Environmental Genomics,J. Craig Venter Institute, 3Donnelly Centre & Department of Molecular Genetics,University of Toronto, 4Lunenfeld Research Institute,Mt Sinai Hospital

Chapters

  • 00:05Title
  • 01:38Sexual Cycling
  • 06:13Flow Cytometry
  • 09:26Genotyping
  • 10:28Results: Yeast Strains Carrying Multiple Gene Deletions as Generated by the Green Monster Process
  • 12:47Conclusion

Summary

Automatic Translation

Automatic Translation

绿色妖怪方法能够快速组装的多个标记的报道基因编码绿色荧光蛋白的缺失。这种方法是基于对驾驶酵母菌株通过各种各样的缺失性和携带更多的缺失的细胞的荧光为基础的浓缩的重复循环。

Tags

Green Monster ProcessYeast StrainsGene DeletionsPhenotypesGenetic BackgroundEnvironmental ConditionMutation TestingHidden Gene FunctionsGenetic InteractionsMulti-mutant InteractionsParalogsSaccharomyces CerevisiaeGene KnockoutSelectable MarkerHomologous RecombinationMarker RemovalGreen Monster MethodGreen Fluorescent Protein (GFP) Reporter

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