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Biology
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用于
老德法纹
状体唾液采集的双层膜夹层法
JoVE Journal
Biology
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JoVE Journal
Biology
Two-layered Membrane Sandwich Method for
Laodelphax striatellus
Saliva Collection
Please note that all translations are automatically generated.
Click here for the English version.
用于
老德法纹
状体唾液采集的双层膜夹层法
DOI:
10.3791/62831-v
•
06:13 min
•
August 27, 2021
•
Jing Zhao
2,3
,
Jie Yang
2,3
,
Lili Zhang
2
,
Rongxiang Fang
2
,
Yan Huo
2
1
State Key Laboratory of Plant Genomics, Institute of Microbiology
,
Chinese Academy of Sciences
,
2
National Plant Gene Research Center
,
3
University of the Chinese Academy of Sciences
Chapters
00:05
Introduction
01:02
Preparation of Feeding Chamber and Artificial Diet
02:00
Collection of Small Brown Planthopper (SBPH) Saliva
02:56
Concentration of the Collected Saliva
03:58
Results: Artificial Feeding and Saliva Collection from
Laodelphax striatellus
05:21
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本方案描述了一种使用人工介质从穿刺-吸吮昆虫中收集足够唾液的方法。这是一种收集昆虫唾液和研究唾液对昆虫进食行为和媒介传播病毒传播的有用方法。
Tags
Membrane Sandwich Method
Laodelphax Striatellus
Saliva Collection
Artificial Feeding
Saprophytic Insects
Sucrose Solution
Plant Hopper Larvae
Feeding Chamber
Paraffin Membrane
Artificial Diet
Aluminum Foil
Sterile Tubes
Centrifugal Filter
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