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Gene Transfer into Older Chicken Embryos by ex ovo Electroporation

Article DOI: 10.3791/4078-v • 06:10 min • July 27th, 2012
July 27th, 2012 •
1, 1, 2, 1
1Albrecht-Kossel-Institute for Neuroregeneration, School of Medicine University of Rostock, 2Institute of Anatomy I, School of Medicine University of Jena

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A method of gene transfer into chicken embryos at later incubation stages (older than Hamburger and Hamilton stage (HH) 22) is described. This method overcomes disadvantages of in ovo electroporation applied to older chicken embryos and is a useful technique to study gene function and regulation at older developmental stages.

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Gene Transfer Chicken Embryos Ex Ovo Electroporation Gene Function Gene Regulation In Vivo DNA Plasmids SiRNA Plasmids RNA Oligos Morpholino Antisense Oligonucleotides Incubation Stages Vitelline Membrane Allantoic Vessels Gene Transfer Protocol
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