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Genoverføring til Eldre Chicken Embryoer av<em> Ex ovo</em> Electroporation
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Gene Transfer into Older Chicken Embryos by ex ovo Electroporation

Genoverføring til Eldre Chicken Embryoer av<em> Ex ovo</em> Electroporation

DOI:
10.3791/4078-v

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06:10 min

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July 27, 2012

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, , ,
1Albrecht-Kossel-Institute for Neuroregeneration,School of Medicine University of Rostock, 2Institute of Anatomy I,School of Medicine University of Jena

Chapters

  • 00:05Title
  • 01:22Ex ovo Culture
  • 02:51Preparation for Ex ovo Electroporation
  • 03:51Gene Transfer into Chicken Optic Tectum by Ex ovo Electroporation
  • 04:43Results: Overexpression of Cad7 and GFP by Ex ovo Electroporation
  • 05:50Conclusion

Summary

Automatic Translation

Automatic Translation

En metode for genoverføring til kylling embryoer ved senere inkubering stadier (eldre enn hamburger og Hamilton scenen (HH) 22) beskrives. Denne metoden overvinner ulempene ved<em> I ovo</em> Electroporation gjaldt eldre kylling embryoer og er en nyttig teknikk for å studere gen-funksjon og regulering på eldre utviklingsstadier.

Tags

Gene TransferChicken EmbryosEx Ovo ElectroporationGene FunctionGene RegulationIn VivoDNA PlasmidsSiRNA PlasmidsRNA OligosMorpholino Antisense OligonucleotidesIncubation StagesVitelline MembraneAllantoic VesselsGene Transfer Protocol

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