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在疾病的染色质蛋白质组的定量分析
JoVE Journal
Medicine
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JoVE Journal Medicine
Quantitative Analysis of Chromatin Proteomes in Disease
DOI:

08:11 min

December 28, 2012

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Chapters

  • 00:05Title
  • 01:06Heart Homogenization and Nuclear Isolation
  • 02:22Nucleoplasm and Detergent-extracted Chromatin Fractionation
  • 03:22Acid-extraction Fractionation – DNA-bound Protein Enrichment
  • 04:51Representative Chromatin Proteome Quantitation
  • 07:49Conclusion

Summary

Automatic Translation

允许在质谱高通量分析蛋白的表达和组织了一系列修改。结合亚细胞的分馏和疾病模型,定量质量法和生物信息学在生物系统中发现新的属性。这里描述的方法分析染色质相关蛋白在心脏疾病的设置和容易地适用于其他<em在体内</em>人类疾病的模型。

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