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1Department of Anesthesiology,David Geffen School of Medicine at UCLA, 2Department of Medicine,David Geffen School of Medicine at UCLA, 3Department of Physiology,David Geffen School of Medicine at UCLA, 4Department of Internal Medicine,Nora Eccles Harrison Cardiovascular Research and Training Institute, University of Utah
Chapters
- 00:05Title
- 01:06Heart Homogenization and Nuclear Isolation
- 02:22Nucleoplasm and Detergent-extracted Chromatin Fractionation
- 03:22Acid-extraction Fractionation – DNA-bound Protein Enrichment
- 04:51Representative Chromatin Proteome Quantitation
- 07:49Conclusion
Tags
Quantitative AnalysisChromatin ProteomesDiseaseNucleusGene RegulationAdult Mammalian Cardiomyocyte NucleiBinucleated CellsHeterochromatic StateDNA PackagingTranscriptional RegulationDevelopmentDiseaseNuclear ProteinsTranscriptional ProgramsHeart DiseaseMortalityNuclear ProteomeMass SpectrometryAnnotationRelative QuantificationProtein AbundanceProteomic StudiesCardiac Nuclear ProteomeNuclear Sub Compartments
