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支气管肺泡灌洗(BAL)的研究,获得适当的采样率
JoVE Journal
Medicine
This content is Free Access.
JoVE Journal
Medicine
Bronchoalveolar Lavage (BAL) for Research; Obtaining Adequate Sample Yield
Please note that all translations are automatically generated.
Click here for the English version.
支气管肺泡灌洗(BAL)的研究,获得适当的采样率
DOI:
10.3791/4345-v
•
11:47 min
•
March 24, 2014
•
Andrea M. Collins
2
,
Jamie Rylance
,
Daniel G. Wootton
,
Angela D. Wright
5
,
Adam K. A. Wright
3
,
Duncan G. Fullerton
6
,
Stephen B. Gordon
1
Biomedical Research Centre in Microbial Diseases
,
National Institute for Health Research
,
2
Respiratory Infection Group
,
Royal Liverpool and Broadgreen University Hospital Trust
,
3
Respiratory Infection Group
,
Liverpool School of Tropical Medicine
,
4
Institute of Infection and Global Health
,
University of Liverpool
,
5
Comprehensive Local Research Network
,
Royal Liverpool and Broadgreen University Hospital Trust
,
6
Department of Respiratory Research
,
University Hospital Aintree
Chapters
00:05
Title
01:56
Preparation for the Procedure
04:32
Insertion and Positioning of the Bronchoscope
05:39
Performing Bronchoalveolar Lavage
07:14
Subject Recovery
08:13
Isolation of Cells from Bronchoalveolar Lavage
10:33
Results: Microscopic Observation of Cells Obtained by Bronchoalveolar Lavage
11:05
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述了使用低压吸入纤维支气管镜检查和支气管肺泡研究技术。该技术被用于从肺支气管肺泡表面收获的免疫细胞。局部麻醉剂和轻度镇静(咪达唑仑)被使用。主题容忍的程序以及经验和最小的副作用。
Tags
Bronchoalveolar Lavage
BAL
Fiberoptic Bronchoscopy
Research Technique
Manual Suction
Nonadherent Cells
Lung Lining Fluid
Mucosal Surface
Innate Responses
Cellular Responses
Humoral Responses
Lung Macrophage
B-cells
T-cells
Immunoglobulin
Volume Instillate
Cell Damage
Local Anesthetic
Conscious Sedation
Tolerance
Side Effects
Written Informed Consent
Inclusion Criteria
Exclusion Criteria
Article
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