A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Department of Stem Cells and Developmental Biology, Cell Science Research Center,Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran, 2Department of Endocrinology & Female Infertility, Reproductive Biomedicine Center,Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran
Chapters
- 00:00Title
- 01:18Introduction
- 03:16Preparation of Enzyme Solution
- 04:06Human Dental Pulp Isolation
- 04:48Isolation of Dental Pulp Stem Cells by Enzymatic Dissociation of Dental Pulp Tissue
- 06:28Outgrowth of Stem Cells from Tissue Explants
- 07:27Immunophenotyping
- 08:26Inducing Odontoblast Differentiation & Alizarin Red Staining Assay
- 12:21Dental Pulp Stem Cells Culture and Differentiation Results
- 14:14Conclusion
Metoden er beskrevet isolering og karakterisering av menneskelig tannpulpa stamceller (hDPSCs) ved hjelp av enten<strong> Enzymatisk dissosiasjon av papirmasse (DPSC-ED)</strong> Eller<strong> Direkte resultat av stamceller fra pulpavevet explants (DPSC-OG). Deretter fulgt av</strong><em> In vitro</em> Sammenlignende differensiering av begge typer hDPSCs inn odontoblaster.
Tags
IsolationCharacterizationComparative DifferentiationHuman Dental Pulp Stem CellsPermanent TeethTwo Different MethodsWisdom TeethRoutine Orthodontic TreatmentsHDPSCsProliferation PotentialMulti-lineage Differentiation CapacityAutologous TransplantationRegenerative MedicineMesenchymal Stem CellsMSC FeaturesImmunomodulatory EffectAllograft TransplantationIsolation ConditionsSurface MarkersDifferentiation CapacityImpacted Third Molar TeethDental Diamond Disk
