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Isolasjon, karakterisering og Comparative Differensiering av Human tannpulpa stamceller hentet fra permanente tenner ved hjelp av to ulike metoder
JoVE Journal
Medicine
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JoVE Journal Medicine
Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods
DOI:

14:52 min

November 24, 2012

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Chapters

  • 00:00Title
  • 01:18Introduction
  • 03:16Preparation of Enzyme Solution
  • 04:06Human Dental Pulp Isolation
  • 04:48Isolation of Dental Pulp Stem Cells by Enzymatic Dissociation of Dental Pulp Tissue
  • 06:28Outgrowth of Stem Cells from Tissue Explants
  • 07:27Immunophenotyping
  • 08:26Inducing Odontoblast Differentiation & Alizarin Red Staining Assay
  • 12:21Dental Pulp Stem Cells Culture and Differentiation Results
  • 14:14Conclusion

Summary

Automatic Translation

Metoden er beskrevet isolering og karakterisering av menneskelig tannpulpa stamceller (hDPSCs) ved hjelp av enten<strong> Enzymatisk dissosiasjon av papirmasse (DPSC-ED)</strong> Eller<strong> Direkte resultat av stamceller fra pulpavevet explants (DPSC-OG). Deretter fulgt av</strong><em> In vitro</em> Sammenlignende differensiering av begge typer hDPSCs inn odontoblaster.

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