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An Enzyme-free Method for Isolation and Expansion of Human Adipose-derived Mesenchymal Stem Cells
JoVE Journal
Medicine
This content is Free Access.
JoVE Journal Medicine
An Enzyme-free Method for Isolation and Expansion of Human Adipose-derived Mesenchymal Stem Cells

An Enzyme-free Method for Isolation and Expansion of Human Adipose-derived Mesenchymal Stem Cells

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04:37 min

December 16, 2019

DOI:

04:37 min
December 16, 2019

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Summary

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This protocol provides an enzyme-free method for isolating mesenchymal stem cells from abdominoplasty and lipoaspirate samples using an explant method. The absence of harsh enzymes or centrifugation steps provides for clinically relevant stem cells that can be used for studies in vitro or transferred back to the clinic.

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