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JoVE Journal
Biology

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E4orf4诱导的细胞死亡的击倒效果的基因表达和测量条件击倒细胞系的制备
 
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E4orf4诱导的细胞死亡的击倒效果的基因表达和测量条件击倒细胞系的制备

Article DOI: 10.3791/4442
October 21st, 2012

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Summary October 21st, 2012

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ACF染色质重塑因子E4orf4诱导的细胞死亡的贡献进行了测量。该协议包括在多西环素治疗诱导有条件击倒的ACF的亚基ACF1和SNF2h的细胞克隆的选择,使用的DAPI检测测量E4orf4-诱导的细胞死亡的诱导的细胞系。

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