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体内转染和基因敲除在脊髓通过快速无创腰椎鞘内注射小鼠
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JoVE Journal Biology
In Vivo SiRNA Transfection and Gene Knockdown in Spinal Cord via Rapid Noninvasive Lumbar Intrathecal Injections in Mice
Please note that all translations are automatically generated. Click here for the English version.

体内转染和基因敲除在脊髓通过快速无创腰椎鞘内注射小鼠

DOI:
10.3791/51229-v

•

05:24 min

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March 22, 2014

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, ,
1Institute for Pharmacology,University of Heidelberg

Chapters

  • 00:05Title
  • 01:20Preparation of siRNA/PEI Complex
  • 02:02Intrathecal Injection
  • 03:46Results: Intrathecal Injections and siRNA Downregulation of Protein
  • 05:00Conclusion

Summary

Automatic Translation

Automatic Translation

这份报告描述了鞘内穿刺针用于siRNA在小鼠脊髓腰段下短持久的浅麻醉局部转染一个简单而快速的方法。

Tags

In VivoSiRNATransfectionGene KnockdownSpinal CordLumbar Intrathecal InjectionMiceNoninvasivePolyethyleniminePEISpinal FunctionsPharmacological AgentsRNA Modulators

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