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体内转染和基因敲除在脊髓通过快速无创腰椎鞘内注射小鼠
JoVE Journal
Biology
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JoVE Journal Biology
In Vivo SiRNA Transfection and Gene Knockdown in Spinal Cord via Rapid Noninvasive Lumbar Intrathecal Injections in Mice
DOI:

05:24 min

March 22, 2014

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Chapters

  • 00:05Title
  • 01:20Preparation of siRNA/PEI Complex
  • 02:02Intrathecal Injection
  • 03:46Results: Intrathecal Injections and siRNA Downregulation of Protein
  • 05:00Conclusion

Summary

Automatic Translation

这份报告描述了鞘内穿刺针用于siRNA在小鼠脊髓腰段下短持久的浅麻醉局部转染一个简单而快速的方法。

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