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Kvantificering Glomerular fyldbarhed Fluorescent Macromolecules Brug 2-Photon mikroskopi i München Wistarrotter
JoVE Journal
Medicine
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JoVE Journal Medicine
Quantifying Glomerular Permeability of Fluorescent Macromolecules Using 2-Photon Microscopy in Munich Wistar Rats
DOI:

11:13 min

April 17, 2013

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Chapters

  • 00:05Title
  • 01:50Exposing the Kidney in a Munich Wistar Rat for Intravital 2-Photon Imaging
  • 04:10Acquiring Images to Quantify Renal Permeability of Albumin
  • 06:02Calculating Glomerular Sieving Coefficient for Fluorescent Albumin from 3D Volumes
  • 08:21Glomerular Permeability Results
  • 10:34Conclusion

Summary

Automatic Translation

En teknik udnytter høj opløsning intavital 2-foton mikroskopi til direkte at visualisere og kvantificere gloemrular filtrering i overfladen glomeruli. Denne metode giver mulighed for direkte bestemmelse af permeabilitetsegenskaber af makromolekyler i både normale og syge tilstande.

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