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Den MultiBac Protein Complex Production Platform på EMBL
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Den MultiBac Protein Complex Production Platform på EMBL

Article DOI: 10.3791/50159-v • 13:51 min • July 11th, 2013
July 11th, 2013 •
1, 1, 1, 1, 1, 1
1EMBL Grenoble Outstation and Unit of Virus Host Cell Interactions (UVHCI) UMR5322

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Protein-komplekser katalyserer vigtige cellulære funktioner. Detaljeret funktionel og strukturel karakterisering af mange essentielle komplekser kræver rekombinant produktion. MultiBac er et baculovirus / insektcelle-systemet særligt skræddersyet til at udtrykke eukaryote proteiner og deres komplekser. MultiBac blev gennemført som en åben adgang platform og standard operationelle procedurer er udviklet til at maksimere sin nytte.

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Molekylær Biologi Genetik bioteknologi Virology Biokemi mikrobiologi Grundlæggende protokoller genomik proteomik Automation laboratorium Bioteknologi multiprotein komplekser Biologisk forskningsdiscipliner robotteknologi Protein komplekser multigene levering rekombinant ekspression baculovirussystem MultiBac platform standardprocedurer (SOP) cell kultur sekventering
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