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Adskillelse af muse embryonale Facial ektoderm og mesenkym
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JoVE Journal Biology
Separation of Mouse Embryonic Facial Ectoderm and Mesenchyme

Adskillelse af muse embryonale Facial ektoderm og mesenkym

DOI:
10.3791/50248-v

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08:36 min

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April 12, 2013

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1Department of Craniofacial Biology,University of Colorado Denver Anschutz Medical Campus, 2Department of Cell and Developmental Biology,University of Colorado Denver Anschutz Medical Campus

Chapters

  • 00:05Title
  • 01:34Dissecting CD-1 E10.5 Embryos
  • 03:18Dispase II Treatment
  • 04:13Separating the Facial Ectoderm and Mesenchyme
  • 06:37Tissue Purity after Separation
  • 07:26Conclusion

Summary

Automatic Translation

Automatic Translation

En protokol til adskillelse af foster facial ektoderm og mesenkym er beskrevet. Vi bruger Dispase II til behandling af hele embryoner først, dissekere hele ansigtet Fremspring ud, og derefter adskille facial ektoderm og mesenkym.

Tags

Facial EctodermFacial MesenchymeOrofacial CleftsFacial ProminencesEmbryonic DevelopmentMouse ModelDispase IIGene ExpressionTranscription FactorsChromatin Marks

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