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嵌入后突触蛋白的免疫标记海马切片培养
 

嵌入后突触蛋白的免疫标记海马切片培养

Article doi: 10.3791/50273
April 3rd, 2013

Summary April 3rd, 2013

Please note that all translations are automatically generated.

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蛋白质的定位和分布提供了重要的信息,了解他们的细胞功能。可以使用电子显微镜(EM)的优越的空间分辨率,以确定一个给定的抗原后免疫组化的亚细胞定位。对于组织的中枢神经系统(CNS),保持结构完整性的同时保持抗原性已经在EM研究特别困难。在这里,我们采用的程序已经被用来保存在中枢神经系统的研究和定性大鼠海马CA1锥体神经元突触蛋白的结构和抗原。

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