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髓鞘有机小脑片培养的制备及免疫染色
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Neuroscience
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JoVE Journal Neuroscience
Preparation and Immunostaining of Myelinating Organotypic Cerebellar Slice Cultures
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髓鞘有机小脑片培养的制备及免疫染色

DOI:
10.3791/59163-v

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09:41 min

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March 20, 2019

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, , , ,
1Sorbonne Université, Inserm, CNRS, Institut du Cerveau et de la Moelle épinière, ICM-GH Pitié-Salpétrière

Chapters

  • 00:04Title
  • 00:36Cerebellum Dissection and Sections Preparation
  • 03:58Slices Culture and Demyelination
  • 04:55Immunohistochemistry
  • 08:15Results: Myelin Immunostaining in Organotypic Cerebellar Slices
  • 09:03Conclusion

Summary

Automatic Translation

Automatic Translation

本文提出了一种利用免疫组织化学方法制备小鼠小脑和髓鞘染色的有机切片培养方法, 该方法适用于研究中枢神经系统髓鞘和再髓鞘的机制。

Tags

Keywords: Myelinating Organotypic Cerebellar Slice CulturesMyelinationRemyelinationCerebellumDissectionImmunostaining

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