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神经血管重塑的分析Entorhino,海马脑切片培养
JoVE Journal
Neuroscience
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Neuroscience
The Analysis of Neurovascular Remodeling in Entorhino-hippocampal Organotypic Slice Cultures
Please note that all translations are automatically generated.
Click here for the English version.
神经血管重塑的分析Entorhino,海马脑切片培养
DOI:
10.3791/52023-v
•
08:27 min
•
October 23, 2014
•
Sophorn Chip
,
Xinzhou Zhu
,
Josef P. Kapfhammer
1
Anatomical Institute, Department of Biomedicine Basel
,
University of Basel
,
2
Department of Neonatology, University Children’s Hospital (UKBB)
,
University of Basel
Chapters
00:05
Title
01:20
Organotypic Entorhino-hippocampal Slice Cultures
02:37
In Vitro Hypoxia and Oxygen-glucose Deprivation (OGD) with Reperfusion
04:28
Fixation and Immunostaining
05:47
Determination of Regional Vessel Density
06:22
Results: Neuronal Death and Blood Vessel Loss after Hypoxia or OGD
07:41
Conclusion
Summary
Automatic Translation
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Automatic Translation
一种协议,用于entorhino,海马脑片培养,允许再生缺血性脑损伤的多个方面,提出了。通过研究,除了在神经血管的变化,以改变神经元,该协议是一个多功能的工具损伤后,研究塑料改变神经组织。
Tags
Brain Ischemia
Neurovascular Remodeling
Organotypic Slice Cultures
Oxygen And Glucose Deprivation
In Vitro Model
Neuronal Damage
Vascular Changes
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