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Metode til mus pancreasø Isolation og intracellulære cAMP Bestemmelse
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Metode til mus pancreasø Isolation og intracellulære cAMP Bestemmelse

Article DOI: 10.3791/50374-v • 12:33 min • June 25th, 2014
June 25th, 2014 •
1, 2, 3, 2
1Department of Nutrional Sciences, University of Wisconsin-Madison, 2Department of Medicine, Division of Endocrinology, Diabetes, and Metabolism, University of Wisconsin-Madison, 3School of Pharmacy, University of Waterloo

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Analysering in vitro β-celle funktion ved hjælp af isolerede mus Langerhanske øer er en vigtig komponent i studiet af diabetes patofysiologi og sygdomsbehandling. Mens mange downstream applikationer er tilgængelige, protokollen specifikt beskriver måling af intracellulær cyklisk adenosinmonophosphat (cAMP) som en væsentlig parameter, β-celle funktion.

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Fysiologi islet isolation insulinsekretion β-celle diabetes cAMP-produktion mus
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