Manufacturing Devices and Instruments for Easier Rat Liver Transplantation

In a Recent video paper on Jo, we described the use of the quick linker device for easier rat liver transplantation. The device is not available on the market, however it can be manufactured without professional skills. In this video, we describe how to design and shape a complete kit starting from common laboratory material.

Enjoy the video. The quick linker kit for orthotopic rat liver transplantation is composed by one approximator, one ring for the repath VNA cava, and a smaller one for the vena portal, secure a number 10 scalpel blade on a wooden board with a tape tuck the center of the ring under the microscope. It should be located 1.2 centimeter straight from the groove of the scalpel.

Mount a diamond bit on a high speed rotating and drill and drill at two millimeters hole. Widen the hole with fuser iconic diamond bits. The whole diameter should be 2.6 millimeters for the portal handle of rat’s wage in between 180 and 240 grams.

Design the external shape of the handle as shown in the stencil. Enclosed in figure one, convert the drill into a mini bench grinder and remove the part of the blade outside the shape. Finally, round of the piece with a low speed rotating brush.

The approximator is obtained by modifying straight surgical climbs or caucus forceps at off half of one branch under the microscope. Using the high speed mini bench grinder, make symmetrical grooves on both sides to allow for the handle to slide in completely. Cut a longitudinal slit into the long arm and check that the center of the long arm and the well positioned handle are on the same axis.

Design a notch on the long arm facing the center of the end. Make the branch as thin as possible. Finally, round of the piece with a low speed rotating brush, rush calf calipers vary according to the rat weights and strains.

However, the land should always be kept around four millimeters. Refer to table one for caliber ranges. Take a fine bo Polyline tubing and round off one edge with a number 10 scalpel.

Design a groove at a 0.5 millimeters from the edge. Now carefully cut out as lo going down to the groove. Cut out one or two full thickness nicks into the tubing.

These will help manipulating the calf during graft preparation and transplantation. Finally cuts the calf at four millimeters from the edge. We showed how to build a kit composed by a ring for the portal vein and its calf, a ring for the hepatic vena cava and its calf and one approximator.

These tools facilitate liver reimplantation by assisting calfs insertion. All metal parts are removed just after fusion, so the kit can be unlimitedly reused no matter what ring size or shape variation you choose. Alignment to the approximator is the most critical factor.

Another important factor is the thickness of the grooves, which shouldn’t be thinner than 0.5 millimeters during, in the bottom of the picture, for example, has been correctly shaped by contrast. In the top one. The metal bridge between two segments is too thin and might lead to a rupture during the phase of final brushing, as in this case following the measures.

Given no problems should occur and the instruments may potentially last forever.