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Visualization and Analysis of mRNA Molecules Using Fluorescence In Situ Hybridization in Saccharomyces cerevisiae
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Visualization and Analysis of mRNA Molecules Using Fluorescence In Situ Hybridization in Saccharomyces cerevisiae

Article DOI: 10.3791/50382 • 07:00 min • June 14th, 2013
June 14th, 2013 •
1,2, 1, 1, 1, 1, 1, 1,3
1The Lewis-Sigler Institute for Integrative Genomics, Princeton University, 2Graduate Program in Quantitative and Computational Biology, Princeton University, 3Department of Molecular Biology, Princeton University

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This protocol describes an experimental procedure for performing Fluorescence in situ Hybridization (FISH) for counting mRNAs in single cells at single-molecule resolution.

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Visualization Analysis MRNA Molecules Fluorescence In Situ Hybridization Saccharomyces Cerevisiae FISH Method Nucleic Acids Native Cellular Environment Protocol Quantification Single Yeast Cells Growth Conditions Fixation Permeability Single-stranded Deoxyoligonucleotides Fluorescent Dyes Labeling Visualization Diffraction-limited Fluorescence Spot-detection Algorithm MRNA Quantification Northern Blots RT-PCR Gene Expression Microarrays Average MRNAs Bulk Population Single-cell Analysis
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