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生产<em>热带爪蟾</em>卵提取物识别微管相关的RNA
JoVE Journal
Biology
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JoVE Journal Biology
Production of Xenopus tropicalis Egg Extracts to Identify Microtubule-associated RNAs
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生产<em>热带爪蟾</em>卵提取物识别微管相关的RNA

DOI:
10.3791/50434-v

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10:01 min

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June 27, 2013

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,
1Department of Molecular Biology,Massachusetts General Hospital, 2Department of Genetics,Harvard Medical School

Chapters

  • 00:05Title
  • 01:15Generation of X. tropicalis Eggs
  • 03:24Preparation of Extract from X. tropicalis Eggs
  • 06:45Purification of Taxol-stabilized Microtubules from X. tropicalis Extract
  • 08:25Representative X. Tropicalis Extraction Results
  • 09:37Conclusion

Summary

Automatic Translation

Automatic Translation

我们描述了收集未受精<em>热带爪蟾</em>鸡蛋和减数分裂II被捕卵提取物的生产。这个卵提取物可用于纯化的微管，微管相关的RNA。

Tags

Xenopus TropicalisEgg ExtractsMicrotubule-associated RNAsTranscript IdentificationHigh-throughput SequencingSubcellular StructuresRNA LocalizationXenopus Laevis

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