A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 01:42Pancreas Dissection and Dilaceration
- 03:51Enzymatic and Mechanical Dissociations of Pancreas
- 05:46Filtration, Seeding of Dispersed Acini, and Acinar Cell Culture
- 07:46Representative Acinar Culturing Results
- 08:23Conclusion
I denne publikation beskriver vi en hurtig og bekvem procedure for isolering og dyrkning primære pancreas acinarceller fra murine bugspytkirtlen. Denne metode udgør en værdifuld tilgang til at studere fysiologi af friske primære normale / utransformeret exokrine bugspytkirtlen celler.
