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小鼠原代胰腺腺泡细胞的分离和培养
JoVE Journal
Biology
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JoVE Journal Biology
Isolation and Culture of Mouse Primary Pancreatic Acinar Cells
DOI:

08:58 min

August 13, 2013

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Chapters

  • 00:05Title
  • 01:42Pancreas Dissection and Dilaceration
  • 03:51Enzymatic and Mechanical Dissociations of Pancreas
  • 05:46Filtration, Seeding of Dispersed Acini, and Acinar Cell Culture
  • 07:46Representative Acinar Culturing Results
  • 08:23Conclusion

Summary

Automatic Translation

在本出版物中,我们描述了一个快速,便捷的程序,用于隔离和培养主要从小鼠胰腺腺泡细胞。这种方法构成一个有价值的方法,以新鲜的正常/未转化的胰腺外分泌细胞的生理研究。

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