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小鼠原代胰腺腺泡细胞的分离和培养

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Biology

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JoVE Journal Biology

Isolation and Culture of Mouse Primary Pancreatic Acinar Cells

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小鼠原代胰腺腺泡细胞的分离和培养

DOI:

10.3791/50514-v

•

08:58 min

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August 13, 2013

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Johann Gout^2,3,4,5, Roxane M. Pommier^2,3,4,5, David F. Vincent^2,3,4,5, Bastien Kaniewski^2,3,4,5, Sylvie Martel^2,3,4,5, Ulrich Valcourt*^1,2,3,4,5, Laurent Bartholin*^1,2,3,4,5

¹INSERM U1052,Centre de Recherche en Cancérologie de Lyon, ²CNRS UMR5286,Centre de Recherche en Cancérologie de Lyon, ³Université de Lyon, ⁴Université Lyon 1, ⁵Centre Léon Bérard

Chapters

00:05Title
01:42Pancreas Dissection and Dilaceration
03:51Enzymatic and Mechanical Dissociations of Pancreas
05:46Filtration, Seeding of Dispersed Acini, and Acinar Cell Culture
07:46Representative Acinar Culturing Results
08:23Conclusion

Summary

Automatic Translation

在本出版物中，我们描述了一个快速，便捷的程序，用于隔离和培养主要从小鼠胰腺腺泡细胞。这种方法构成一个有价值的方法，以新鲜的正常/未转化的胰腺外分泌细胞的生理研究。

Tags

Mouse Pancreatic Acinar Cells Isolation Culture Exocrine Pancreas In Vitro Cell Lines Tumor Differentiation

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