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Patch Clamp Recordings from Embryonic Zebrafish Mauthner Cells
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Neuroscience
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JoVE Journal Neuroscience
Patch Clamp Recordings from Embryonic Zebrafish Mauthner Cells

Patch Clamp Recordings from Embryonic Zebrafish Mauthner Cells

DOI:
10.3791/50551-v

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07:38 min

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September 10, 2013

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,
1Department of Biological Sciences,University of Alberta

Chapters

  • 00:05Title
  • 01:18Preparation and Dissection
  • 03:35Whole-cell Patch Clamp Recording
  • 05:43Results: Patch Clamp Recordings from Embryonic Zebrafish Mauthner Cells
  • 07:11Conclusion

Summary

Automatic Translation

Automatic Translation

We have developed an intact brain-spinal cord preparation to record and monitor electrical activity via patch clamp recording from the Mauthner neurons and other reticulospinal cells in zebrafish embryos. Thus, we are able to record excitatory and inhibitory synaptic currents, voltage-gated channel activity and action potentials from key neurons in a developing embryo.

Tags

Keywords: Mauthner CellsEmbryonic ZebrafishPatch Clamp RecordingsC-start ResponseSynaptic DevelopmentExcitatory Synaptic CurrentsInhibitory Synaptic CurrentsAMPANMDAGABAGlycineVertebrate PreparationSynaptic PlasticityNeurodevelopment

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