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Identifisere DNA Mutasjoner i Purified Hematopoietic Stem / stamceller
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Identifying DNA Mutations in Purified Hematopoietic Stem/Progenitor Cells
DOI:

11:06 min

February 24, 2014

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Chapters

  • 00:05Title
  • 01:45Isolation of Genomic DNA
  • 04:10Packaging of Genomic DNA
  • 05:21Plating Packaged Genomic DNA
  • 07:07Determination of a Putative Mutant Frequency
  • 08:37Results: The in vivo Mutagenesis Assay
  • 10:35Conclusion

Summary

Automatic Translation

Her beskriver vi en in vivo-mutagenese assay for små antall av hematopoetiske celler renset ved hjelp av laci transgen musemodell. Den laci-genet kan bli isolert for å bestemme frekvensen, plasseringen og typen av DNA-mutanter spontant oppstått eller etter eksponering for genotoxins.

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