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Identifisere DNA Mutasjoner i Purified Hematopoietic Stem / stamceller
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1Greehey Children’s Cancer Research Institute,UT Health Science Center at San Antonio, 2Department of Cellular and Structural Biology,UT Health Science Center at San Antonio, 3Department of Pathology,UT Health Science Center at San Antonio, 4Department of Microbiology,UT Health Science Center at San Antonio, 5Cancer Therapy and Research Center,UT Health Science Center at San Antonio
Chapters
- 00:05Title
- 01:45Isolation of Genomic DNA
- 04:10Packaging of Genomic DNA
- 05:21Plating Packaged Genomic DNA
- 07:07Determination of a Putative Mutant Frequency
- 08:37Results: The in vivo Mutagenesis Assay
- 10:35Conclusion
Her beskriver vi en in vivo-mutagenese assay for små antall av hematopoetiske celler renset ved hjelp av laci transgen musemodell. Den laci-genet kan bli isolert for å bestemme frekvensen, plasseringen og typen av DNA-mutanter spontant oppstått eller etter eksponering for genotoxins.
