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光学检测<em> E。大肠杆菌</em>由细菌介孔硅生物传感器
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Optical Detection of E. coli Bacteria by Mesoporous Silicon Biosensors
DOI:

07:22 min

November 20, 2013

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Chapters

  • 00:05Title
  • 01:28Preparation of Fluorescently Labeled, Biofunctionalized, Oxidized Porous Silicon Scaffolds
  • 03:43Bacteria Culture and Bacteria Sensing
  • 05:12Results: A Label-free Optical Detection of E. coli Bacteria
  • 06:55Conclusions

Summary

Automatic Translation

一个无标记光学生物传感器快速检测细菌的介绍。该生物传感器是基于纳米结构的多孔硅,被设计成直接捕获目标细菌的细胞在其表面上。我们使用的单克隆抗体,固定在所述多孔性换能器,作为捕获探针。我们的研究表明这些生物传感器用于与事先没有样品处理(如细胞裂​​解)分钟内低细菌浓度的检测的适用性。

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