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与器官型脑片和癌细胞的3D球体共培养系统
JoVE Journal
Medicine
This content is Free Access.
JoVE Journal
Medicine
Coculture System with an Organotypic Brain Slice and 3D Spheroid of Carcinoma Cells
Please note that all translations are automatically generated.
Click here for the English version.
与器官型脑片和癌细胞的3D球体共培养系统
DOI:
10.3791/50881-v
•
07:48 min
•
October 09, 2013
•
Han-Ning Chuang
,
Raphaela Lohaus
,
Uwe-Karsten Hanisch
,
Claudia Binder
,
Faramarz Dehghani*
3
,
Tobias Pukrop*
1
1
Department of Hematology and Oncology
,
University of Göttingen
,
2
Institute of Neuropathology
,
University of Göttingen
,
3
Institute of Anatomy and Cellbiology
,
University of Halle
Chapters
00:05
Title
01:41
Organotypic Brain Slice Coculturing
04:11
Immunofluorescence and Imaging of Astrocytes and Microglia
05:42
Representative Coculturing Results
07:11
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在器官型脑切片的共培养与肿瘤细胞使肿瘤细胞侵袭的脑组织的过程中可视的形态变化用荧光以及明场(视频)显微镜。这个模型系统还允许细胞交换和补充的方法,并提供多种操作和分析。
Tags
Coculture
Organotypic Brain Slice
3D Spheroid
Carcinoma Cells
Cerebral Metastasis
Tumor Microenvironment
Tumor-associated Macrophages (TAM)
Astrocytes
In Vitro Model
Brain Colonization
Glial Reactions
Molecular Analysis
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