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JoVE Journal Immunology and Infection
Isolamento della corticale Microglia con Immunofenotipo conservato e funzionalità dal murini neonati
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Isolamento della corticale Microglia con Immunofenotipo conservato e funzionalità dal murini neonati

Article DOI: 10.3791/51005-v • 09:12 min • January 30th, 2014
January 30th, 2014 •
1, 1, 1
1Department of Neuroscience, Georgetown University Medical Center

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Una chiave per il successo indagini di biologia microglia è la conservazione dei immunofunction microglia ex vivo durante l'isolamento dal tessuto del sistema nervoso centrale. Isolare microglia via rotanti risultati si stringono in colture cellulari altamente puri e immunofunctional come valutato da immagini fluorescenti, immunocitochimica, ed ELISA dopo l'attivazione della microglia con il lipopolisaccaride stimoli pro-infiammatori (LPS) e Pam 3 CSK 4 (Pam).

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Immunologia neuroinfiammazione citochine neurodegenerazione LPS Pam3CSK4 TLR PAMPs smorza
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