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Immunology and Infection

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JoVE Journal Immunology and Infection
Isolering af Cortical Mikroglia med bevarede immunofænotype og funktionalitet fra murine Nyfødte
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Isolering af Cortical Mikroglia med bevarede immunofænotype og funktionalitet fra murine Nyfødte

Article DOI: 10.3791/51005-v • 09:12 min • January 30th, 2014
January 30th, 2014 •
1, 1, 1
1Department of Neuroscience, Georgetown University Medical Center

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En af nøglerne til en vellykket efterforskning af microglial biologi er bevarelsen af microglial immunofunction ex vivo under isolation fra CNS-væv. Isolerende microglia via roterende rystning resulterer i stærkt rene og immunofunktionel cellekulturer som vurderet af fluorescerende billeddannelse, immuncytokemi, og ELISA efter mikroglia aktivering med det proinflammatoriske stimuli (LPS) og Pam 3 CSK 4 (Pam).

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Immunologi neuroinflammation cytokiner neurodegeneration LPS Pam3CSK4 TLRs PAMPs dæmper
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