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JoVE Journal
Immunology and Infection

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皮层小胶质细胞的分离与保存免疫表型和功能从新生儿鼠
 
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皮层小胶质细胞的分离与保存免疫表型和功能从新生儿鼠

Article DOI: 10.3791/51005-v 09:12 min January 30th, 2014
January 30th, 2014

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Summary

Please note that all translations are automatically generated.

Click here for the English version.

一键小胶质细胞生物学的研究成功是小胶质细胞免疫功能的体外从中枢神经系统组织分离过程中的保存。通过旋转振动造成的高纯度和immunofunctional细胞培养物中分离的小胶质细胞所评估的荧光成像,免疫细胞化学,ELISA和以下的小胶质细胞活化的促炎性刺激脂多糖(LPS)和Pam 3 CSK 4(PAM)。

Tags

免疫学,第83,神经炎症,细胞因子,神经退行性疾病,脂多糖,PAM3CSK4,Toll样受体,的PAMP,DAMPS
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