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Fluorescens<em> In situ</em> Hybridiseringer (FISH) til lokalisering af vira og Endosymbiotic Bakterier i plante-og insektliv Væv
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Fluorescence in situ Hybridizations (FISH) for the Localization of Viruses and Endosymbiotic Bacteria in Plant and Insect Tissues
DOI:

10:38 min

February 24, 2014

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Chapters

  • 00:05Title
  • 01:26Whitefly, Plant, and Virus Preparations
  • 02:10Whole Mount FISH of Whiteflies
  • 03:58Whitefly Dissection Followed by TYLCV FISH
  • 06:40Plant Handling, Hand-sectioning, Fixation, and Hybridization for TYLCV FISH
  • 08:29Results: FISH Images
  • 10:16Conclusion

Summary

Automatic Translation

Vi beskriver her en simpel fluorescens in situ hybridisering (FISH) metode til lokalisering af vira og bakterier i insekt-og plantevæv. Denne protokol kan udvides til visualisering af mRNA i hele mount og mikroskopiske sektioner.

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