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Fluorescens<em> In situ</em> Hybridiseringer (FISH) til lokalisering af vira og Endosymbiotic Bakterier i plante-og insektliv Væv
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Immunology and Infection
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JoVE Journal Immunology and Infection
Fluorescence in situ Hybridizations (FISH) for the Localization of Viruses and Endosymbiotic Bacteria in Plant and Insect Tissues

Fluorescens<em> In situ</em> Hybridiseringer (FISH) til lokalisering af vira og Endosymbiotic Bakterier i plante-og insektliv Væv

DOI:
10.3791/51030-v

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10:38 min

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February 24, 2014

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, , , , , , , , ,
1Department of Entomology,Volcani Center, 2Institute of Plant Sciences and Genetics in Agriculture, Robert H. Smith Faculty of Agriculture, Food and Environment,Hebrew University of Jerusalem, 3Department of Applied Sciences,Institute for Adriatic Crops and Karst Reclamation, 4The Institute of Plant Sciences,Volcani Center

Chapters

  • 00:05Title
  • 01:26Whitefly, Plant, and Virus Preparations
  • 02:10Whole Mount FISH of Whiteflies
  • 03:58Whitefly Dissection Followed by TYLCV FISH
  • 06:40Plant Handling, Hand-sectioning, Fixation, and Hybridization for TYLCV FISH
  • 08:29Results: FISH Images
  • 10:16Conclusion

Summary

Automatic Translation

Automatic Translation

Vi beskriver her en simpel fluorescens in situ hybridisering (FISH) metode til lokalisering af vira og bakterier i insekt-og plantevæv. Denne protokol kan udvides til visualisering af mRNA i hele mount og mikroskopiske sektioner.

Tags

Fluorescence In Situ Hybridization (FISH)Virus LocalizationEndosymbiotic BacteriaSpatial LocalizationVisualizationInfection ProcessReporter Gene SystemsImmunohistochemical MethodsRNA/DNA ProbeFixationHybridizationMicroscopic Visualization

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