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Optical Monitoring of Living Nerve Terminal Labeling in Hair Follicle Lanceolate Endings of the Ex Vivo Mouse Ear Skin
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Optical Monitoring of Living Nerve Terminal Labeling in Hair Follicle Lanceolate Endings of the Ex Vivo Mouse Ear Skin

Optical Monitoring of Living Nerve Terminal Labeling in Hair Follicle Lanceolate Endings of the Ex Vivo Mouse Ear Skin

DOI:
10.3791/53855-v

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11:29 min

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April 05, 2016

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1School of Medical Sciences,University of Aberdeen, 2School of Biological & Biomedical Sciences,University of Durham

Chapters

  • 00:05Title
  • 01:03Preparing Ears for Labeling
  • 03:13Lanceolate Terminal Labeling
  • 06:30Imaging Labeled Lanceolate Endings
  • 08:34Results: Labeled Nerve Terminal in Hair Follicle Lanceolate Endings
  • 10:23Conclusion

Summary

Automatic Translation

Automatic Translation

Here we describe a novel preparation for imaging live lanceolate sensory terminals of palisade endings that innervate mouse ear skin hair follicles during staining and destaining with styryl pyridinium dyes.

Tags

Keywords: Optical MonitoringLiving Nerve TerminalHair Follicle Lanceolate EndingsEx Vivo Mouse Ear SkinMechanosensory Nerve TerminalsSynaptic-like VesiclesCalcium DependenceSkin SeparationCartilage RemovalVital StainingDissection Technique

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