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原位胶质母细胞瘤小鼠模型的维护脑实质的物理约束,并适用于活体双光子显微镜
JoVE Journal
Medicine
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JoVE Journal
Medicine
An Orthotopic Glioblastoma Mouse Model Maintaining Brain Parenchymal Physical Constraints and Suitable for Intravital Two-photon Microscopy
Please note that all translations are automatically generated.
Click here for the English version.
原位胶质母细胞瘤小鼠模型的维护脑实质的物理约束,并适用于活体双光子显微镜
DOI:
10.3791/51108-v
•
09:52 min
•
April 21, 2014
•
Clément Ricard
2
,
Fabio Stanchi
3
,
Geneviève Rougon
2
,
Franck Debarbieux
2
1
Developmental Biology Institute of Marseille
,
Aix Marseille University
,
2
European Research Center for Medical Imaging, Campus de la Timone
,
3
Vesalius Research Center
,
KU Leuven Campus Gasthuisberg
Chapters
00:05
Title
01:26
Craniotomy
03:48
Spheroid Preparation and Implantation
04:58
Closing the Craniotomy with a Window
07:59
Tissue Imaging
09:20
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们已经建立了原位皮质胶质母细胞瘤模型小鼠的活体双光子显微镜的概括了生物物理的限制,通常在作怪肿瘤的生长过程。一种慢性玻璃窗替换上面的肿瘤颅骨使后续的肿瘤进展时间的推移由双光子显微镜。
Tags
Glioblastoma
Mouse Model
Orthotopic
Two-photon Microscopy
Brain Parenchyma
Tumor Spheroid
Dextran Gel
Craniotomy
Fluorescent Transgenic
Intravital Imaging
Neuro-oncology
Drug Testing
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