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Analyse af translationsinitieringskodon under stress betingelser ved Polysomprofiler Profiling
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Analyse af translationsinitieringskodon under stress betingelser ved Polysomprofiler Profiling

Article DOI: 10.3791/51164-v • 10:59 min • May 19th, 2014
May 19th, 2014 •
*1,2, *1,2, 1,2
1Department of Molecular Biology, Medical Biochemistry, and Pathology, Faculty of Medicine, Laval University, 2CHU de Quebec Research Center
* These authors contributed equally

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Her beskriver vi en metode til at analysere ændringer i initiering af mRNA-translation af eukaryote celler som reaktion på stress-tilstande. Denne metode er baseret på hastigheden separation på saccharosegradienter med oversættelse ribosomer fra ikke-translating ribosomer.

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Cellebiologi Oversættelse indvielse polysom ​​profil sucrosegradient protein og RNA isolation stress betingelser
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